Co-immobilization of d-hydantoinase and d-carboamylase on Chitin: Application to the Synthesis of p-hydroxyphenylglycine

Abstract

The immobilization of a multi-enzyme extract is an excellent method for multi-step biotransformations. This paper describes how a multi-enzyme extract from Agrobacterium radiobacter, rich in d-hydantoinase and N-carbamyl-d-amino acid amidohydrolase was immobilized on chitin for its application on the synthesis of p-hydroxyphenylglycine. The adsorption derivative showed a higher activity than the covalent one. Compared to the soluble multi-enzymatic extract, the adsorption derivative showed greater pH-stability in the pH range under study. Its optimum pH ranged from 7–8. Furthermore, it showed high activity at low temperature.

• Agrobacterium radiobacter
• chitin
• co-immobilization
• d-Hydantoinase
• N-Carbamyl-d-Amino acid Amidohydrolase