cDNA Cloning of Translationally Controlled Tumor Protein/Histamine Releasing Factor (TCTP/HRF) from the Intertidal Harpacticoid Copepod Tigriopus japonicus

Il-Chan Kim Department of Biochemistry, College of Medicine, Hanyang University, 133-791, Seoul, South Korea

Young Ja Kim Department of Environmental Science, Graduate School, Hanyang University, 133-791, Seoul, South Korea

Young-Mi Lee Department of Environmental Science, Graduate School, Hanyang University, 133-791, Seoul, South Korea

Bok-Geon Kim Department of Life Science, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea

Tae-Jin Park Department of Life Science, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea

Hyeung-Sin Kim Research Institute for Basic Science, Cheju National University, 690-756, Jeju, South Korea

Min-Min Jung Nam-Jeju Hatchery, South Sea Regional Fisheries Research Institute, National Fisheries Research and Development Institute, 699-800, Jeju, South Korea

Tim D. Williams School of Biosciences, The University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK

Wonchoel Lee Department of Life Science, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea

Jae-Seong Lee Department of Environmental Science, Graduate School, Hanyang University, 133-791, Seoul, South KoreaCorrespondence[email protected]

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Abstract

We synthesized a cDNA library from the intertidal copepod Tigriopus japonicus, converted it to phagemids and sequenced expressed sequence tags (ESTs). Of these, Tigriopus translationally controlled tumor protein/histamine releasing factor (TCTP/HRF) was further characterized. The Tigriopus TCTP/HRF gene encoded 172 amino acid residues and showed high similarity to Drosophila but moderate similarity to other annelids (e.g. Brugia, Wuchereria and C. elegans). The Tigriopus TCTP/HRF gene appeared in the same clade as the annelids. Here, we describe the analysis of the Tigriopus TCTP/HRF gene.

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cDNA Cloning of Translationally Controlled Tumor Protein/Histamine Releasing Factor (TCTP/HRF) from the Intertidal Harpacticoid Copepod Tigriopus japonicus

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cDNA Cloning of Translationally Controlled Tumor Protein/Histamine Releasing Factor (TCTP/HRF) from the Intertidal Harpacticoid Copepod Tigriopus japonicus

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