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Abstract
There are four LhcII genes of Dunaliella salina have been submitted to the database of GenBank. However, little is known about Lhca genes of this green alga, although this knowledge might be available to study the composition and phylogenesis of Lhc gene family. Recently, one Lhca gene was been cloned from the green alga D. salina by PCR amplification using degenerate primers. This cDNA, designated as DsLhca1, contains an open reading frame encoded a protein of 222 amino acids with a calculated molecular mass of 27.8 kDa. DsLhca1 is predicted to contain three transmembrane domains and a N-terminal chloroplast transit peptide (cTP) with length of 33 amino acids. The genomic sequence of DsLhca1 is composed of five introns. The deduced polypeptide sequence of this gene showed a lower degree of identity (less than 30%) with LHCII proteins from D. salina. But its homology to Lhca proteins of other algae (Volvox carteri Lhca_AF110786) was higher with pairwise identities of up to 67.1%. Phylogenetic analysis indicated that DsLhca1 protein cannot be assigned to any types of Lhca proteins in higher plants or in Chlamydomonas reinhardtii.
| Abbreviations | ||
| Chl | = | chlorophyll(s) |
| LHC | = | light-harvesting complex(es) |
| LHCI | = | light-harvesting complex of PS I |
| LHCII | = | light-harvesting complex of PS II |
| Lhcb genes | = | genes coding for LHC proteins in PSII |
| Lhca genes | = | genes coding for LHCI proteins |
| PS I | = | photosystem I (II) |
| PS II | = | photosystem II |
| TMH | = | transmembrane helix (helices) |
| Abbreviations | ||
| Chl | = | chlorophyll(s) |
| LHC | = | light-harvesting complex(es) |
| LHCI | = | light-harvesting complex of PS I |
| LHCII | = | light-harvesting complex of PS II |
| Lhcb genes | = | genes coding for LHC proteins in PSII |
| Lhca genes | = | genes coding for LHCI proteins |
| PS I | = | photosystem I (II) |
| PS II | = | photosystem II |
| TMH | = | transmembrane helix (helices) |
Acknowledgements
This work was supported by the National Natural Science Foundation of China (30470928, 30270711, 30500287).