The narQP genes for a two-component regulatory system from the deep-sea bacterium Shewanella violacea DSS12

Abstract

Shewanella violacea DSS12 is facultative piezophile isolated from the deep-sea. The expression of cydDC genes (required for d-type cytochrome maturation) of the organism is regulated by hydrostatic pressure. In this study, we analyzed the nucleotide sequence upstream of cydDC in detail and found that there are putative binding sites for the NarL protein which is part of a two-component regulatory system also containing the sensor protein NarX. Furthermore, we identified the narQP genes (homologues of narXL) from S. violacea DSS12 and demonstrated the heterologous expression of narP in Escherichia coli. These results will be helpful in examining pressure regulation of gene expression in S. violacea at the molecular level.

• Shewanella violacea
• narP
• respiratory system
• pressure-regulation of gene expression
• two-component regulatory system

Acknowledgements

We thank Dr Howard. K. Kuramitsu for assistance in editing the manuscript.