Possible involvement of orphan receptors GPR88 and GPR124 in the development of hypertension in spontaneously hypertensive rat

ABSTRACT

Hypertension (HBP) is a chronic disease characterized by increased blood pressure, which despite several treatments maintains a high morbi-mortality, which suggests that there are other mechanisms involved in this pathology, within which the orphan receptors could be candidates for the treatment of the HBP; these receptors are called orphan receptors because their ligand is unknown. These receptors have been suggested to participate in some pathologies because they are associated with various systems such as GPR88, which has been linked to the dopaminergic system, and GPR124 with angiogenesis, suggesting that these receptors could take part in HBP. Hence, the aim of this work was to study the expression of orphan receptors GPR88 and GPR124 in various tissues of normotensive and hypertensive rats. We used Wistar Kyoto (WKY) and spontaneously hypertensive rat (SHR) of 6–8 and 10–12 weeks of age and we determined systolic blood pressure (SBP), heart rate, as well as mRNA of GPR88 and GPR124 receptors by reverse transcription polymerase chain reaction (RT-PCR) in the aorta, heart, kidney, and brain. Our results showed that GPR88 and GPR124 were expressed in all analyzed tissues, but their expression is dependent on the age and development of HBP because their expression tends to be modified as HBP is established. Therefore, we conclude that GPR88 and GPR124 receptors may be involved in the development or maintenance of high blood pressure.

KEYWORDS:

• Cardiovascular
• GPR88
• GPR124
• hypertension
• onorphan
• receptors

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

Funding

This work was supported by grants CONACYT CB-1012-01-183660 (Consejo Nacional de Ciencia y Tecnología) CONACYT, project SIP-IPN-20161519 (Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional), and CONACYT scholarship to the student Loranda Calderon Zamora (230286).

Additional information

Funding

This work was supported by grants CONACYT CB-1012-01-183660 (Consejo Nacional de Ciencia y Tecnología) CONACYT, project SIP-IPN-20161519 (Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional), and CONACYT scholarship to the student Loranda Calderon Zamora (230286).