Green synthesis and characterisation of silver nanoparticles from leaf and bark extract of Croton macrostachyus for antibacterial activity

ABSTRACT

In the advanced progress of the synthesis of metal nanoparticles, the use of a bio-mimicking approach has been extensively studied and recognised as a non-toxic method. The use of Ag-NPs synthesised from inorganic compounds using plant extracts as surfactants are quite novel. Therefore, this study was carried out by synthesising and characterising Ag-NPs using a composite leave and bark extract solution of Croton macrostachyus as a capping agent. A 1:3 composite solution exhibited small nanoparticles with a rod-like shape that were active against bacteria. In addition, Ag-NPs were observed from leaf and bark extracts of a 1:1 components, as well as sand mall NPs from leaf extract, showing that leaf extract solution contained more reducing biomolecules. Furthermore, we studied the antibacterial activity of the synthesised NPs following the disc diffusion method (by preparing gram-negative and gram-positive bacteria). Thus, the synthesised NPs were active on Streptococcus pyogen compared with Pseudomonas aeruginosa bacteria.

KEYWORDS:

• Green synthesis
• Croton macrostachyus
• silver nanoparticles
• antimicrobial activity

Acknowledgments

The authors would like to acknowledge chemistry department, Wolkite University, Ethiopia for financial support, Adama Science and Technology University, Ethiopia for allowing us to use XRD and SEM instruments.

Disclosure statement

The authors declare that they have no known competing financial interests or personal relationships that could influence the work reported in this paper.