Green synthesis of biofabricated silver nanoparticles from Syzygium aromaticum seeds: spectral characterization and evaluation of its anti-mycobacterial activity, cytotoxicity assessment on zebrafish embryo and Artemia salina

ABSTRACT

The lack of a viable vaccine and the emergence of novel Mycobacterium tuberculosis (MTB) strains that are particularly resistant to treatments, presage a complicated future situation. Biosynthesized nanomaterials are currently proving to be a viable antibacterial therapeutic option, including for MTB infection treatment. The goal of this work is to synthesize silver nanoparticles (AgNPs) from Syzygium aromaticum seeds and investigate their antimicrobial, anti-tubercular, and cytotoxic properties using zebrafish embryos and Artemia salina. The UV spectrophotometer, SEM-EDAX, TEM and FTIR measurements were used to characterize the AgNPs. Antibacterial activity was performed against S. aureus, P. aeruginosa, E. coli and K. pneumoniae and exhibited potential inhibitory activity towards the bacterial cultures. The MABA assay was used to investigate the anti-mycobacterial activity, and the AgNPs showed the highest percentage of inhibition in both test concentrations (250 and 500  µg/ml).

KEYWORDS:

• Tuberculosis
• silver nanoparticles
• pseudomonas aeruginosa
• Escherichia coli
• Klebsiella pneumoniae
• Artemia salina

Acknowledgments

The authors are thankful to the Researchers Supporting Project number (RSPD2023R728), King Saud University, Riyadh, Saudi Arabia.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article.