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Abstract
Hemin has been reported to be protective in the pathological process, but its protective mechanisms have not been precisely defined. Hemin could induce Erk1/2 phosphorylation in astrocyte. Erk1/2 phosphorylation has been proved to be involved in many growth signals cellular transduction. However, little study has been conducted as to the relationship between hemin and Erk1/2 activation in human umbilical vein endothelial cells (HUVECs). The present study aimed to investigate the relationship between hemin and Erk1/2 phosphorylation in HUVECs. The results showed that low concentration of hemin induced and sustained phosphorylation of Erk1/2 for a long time. The HO inhibitor protoporphyrin IX zinc (II) abrogated phosphorylation of Erk1/2 induced by hemin. Biliverdin, one of the metabolites of hemin, obviously induced the Erk1/2 phosphorylation in HUVECs. Both hemin and biliverdin promoted HUVEC cell growth. The results strongly suggested that hemin could induce and sustain Erk1/2 phosphorylation in HUVECs by way of HO-1 induction and biliverdin produced from HO-1 catalysing hemin degradation.
| Abbreviations | ||
| HUVECs | = | human umbilical vascular endothelial cells |
| HO-1 | = | heme oxygenase-1 |
| ECs | = | endothelial cells |
| H2O2 | = | hydrogen peroxide |
| ROS | = | reactive oxygen species |
| MAPK | = | mitogen-activated protein kinases |
| ZnPP | = | protoporphyrin IX zinc (II) |
| Erk1/2 | = | extracellular signal regulated kinases 1/2 |
| Hb | = | haemoglobin |
| DFO | = | deferoxamine mesylate salt |
| CORM | = | tricarbonyldichlororuthenium (II) dimer (a kind of exterior carbon monoxide-releasing molecule) |
| FAC | = | ferric ammonium citrate |
| ECL | = | chemiluminescence detection |
| BrdU | = | Bromodeoxyuridine |
| Abbreviations | ||
| HUVECs | = | human umbilical vascular endothelial cells |
| HO-1 | = | heme oxygenase-1 |
| ECs | = | endothelial cells |
| H2O2 | = | hydrogen peroxide |
| ROS | = | reactive oxygen species |
| MAPK | = | mitogen-activated protein kinases |
| ZnPP | = | protoporphyrin IX zinc (II) |
| Erk1/2 | = | extracellular signal regulated kinases 1/2 |
| Hb | = | haemoglobin |
| DFO | = | deferoxamine mesylate salt |
| CORM | = | tricarbonyldichlororuthenium (II) dimer (a kind of exterior carbon monoxide-releasing molecule) |
| FAC | = | ferric ammonium citrate |
| ECL | = | chemiluminescence detection |
| BrdU | = | Bromodeoxyuridine |