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Abstract
The family of NADPH oxidase (NOX) genes produces reactive oxygen species (ROS) pivotal for both cell signalling and host defense. To investigate whether NOX and NOX accessory gene expression might be a factor common to specific human tumour types, this study measured the expression levels of NOX genes 1–5, dual oxidase 1 and 2, as well as those of NOX accessory genes NoxO1, NoxA1, p47phox, p67phox and p22phox in human cancer cell lines and in tumour and adjacent normal tissue pairs by quantitative, real-time RT-PCR. The results demonstrate tumour-specific patterns of NOX gene expression that will inform further studies of the role of NOX activity in tumour cell invasion, growth factor response and proliferative potential.
| Abbreviations | ||
| NOX | = | NADPH oxidase |
| Duox | = | dual oxidase |
| HI-FCS | = | heat-inactivated foetal calf serum |
| H2O2 | = | hydrogen peroxide |
| = | superoxide | |
| ROS | = | reactive oxygen species |
| RT-PCR | = | reverse transcriptase-polymerase chain reaction |
| VSMC | = | vascular smooth muscle cell |
| Abbreviations | ||
| NOX | = | NADPH oxidase |
| Duox | = | dual oxidase |
| HI-FCS | = | heat-inactivated foetal calf serum |
| H2O2 | = | hydrogen peroxide |
| = | superoxide | |
| ROS | = | reactive oxygen species |
| RT-PCR | = | reverse transcriptase-polymerase chain reaction |
| VSMC | = | vascular smooth muscle cell |
Supplementary Fig S1–S13. mRNA expression of NOX isoforms and accessory genes in human tumours and adjacent non-malignant tissues relative to 18S rRNA levels. Of 237 clinical samples from 13 different malignancies, equal numbers of tumour and adjacent non-malignant tissue specimens were obtained from 14 patients with moderately- to poorly-differentiated colorectal adenocarcinoma (Figure 1), 14 patients with moderately- to poorly-differentiated non-small cell lung cancer (Figure 2), eight patients with primary hepatocellular cancer (Figure 3), eight patients with moderately- to poorly-differentiated renal cell cancer (Figure 4), 17 patients with moderately- to poorly-differentiated prostate adenocarcinoma (Figure 5), nine patients with moderately- to poorly-differentiated gastric adenocarcinoma (Figure 6), 12 patients with moderately- to poorly-differentiated infiltrating ductal carcinoma of the breast (Figure 8) and 10 patients with moderately- to poorly-differentiated testicular cancer (Figure 9). NOX isoform expression is shown for tumour samples only from patients with: moderately- to poorly-differentiated ovarian adenocarcinoma (12 tumour samples, Figure 7), moderately- to poorly-differentiated squamous carcinoma of the head and neck (nine tumour samples, Figure 10), malignant melanoma (13 tumour samples, Figure 11), five samples of chronic myelogenous leukaemia in relapse (Figure 12) and five brain tumours of glioblastoma multiforme sub-type (Figure 13). The horizontal bars in the figures represent the mean values for gene expression relative to 18S rRNA (×10−8).