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Original

Investigation of albumin properties in patients with chronic renal failure

, , &
Pages 1008-1018 | Received 16 Feb 2009, Published online: 15 Sep 2009
 

Abstract

The aim of this study was the investigation of HSA properties and its structural changes after modification induced in vivo among patients with CRF who underwent haemodialysis. Application of different fluorescent dyes allowed the investigation of different regions of albumin molecule using ANS, bis-ANS, piren, piren maleimide and fluorescein isothiocyanate. As markers of oxidative modification, the total protein thiol, carbonyls, glycosylated plasma proteins and hydroperoxide were estimated in plasma. Additionally, this study investigated plasma viscosity and total antioxidant capacity (TAC) of the plasma. Results show that haemodialysis provoked significant changes in conformational properties of plasma albumin, which resulted in the loss of its biological functions. These findings suggest that oxidative stress and glycation of proteins in plasma are developed during haemodialysis. The results depict that one of the features of uraemia is the presence of signs of oxidative stress before haemodialysis. Nevertheless, oxidative stress and glycation of proteins in plasma are exacerbated during haemodialysis and are a complex process.

Abbreviations
CRD=

carbonyl reactive derivatives

RCD=

reactive carbonyl derivatives

AOPP=

advanced oxidation protein products

HAS=

human serum albumin

CRF=

chronic renal failure

HD=

haemodialysis

EPR=

electron paramagnetic resonance

ANS=

ANS-1-anilino-8-naphthalenesulfonic acid

bis-ANS=

4,4′-Bis(1-anilino-8-naphtalenesulfonic acid)

=

dipotassium salt

FITC=

fluorescein isothiocyanate

DPPH=

1,1-diphenyl-2-picrylhydrazyl

DTNB=

5,5′-dithio-bis (2-nitrobenzoic acid)

RBC=

red blood cell

ROS=

reactive oxygen species

TAC=

total antioxidant capacity

SOD=

superoxide dismutase

PBS=

phosphate buffered saline

MDA=

malondialdehyde

NaCl=

sodium chloride

Abbreviations
CRD=

carbonyl reactive derivatives

RCD=

reactive carbonyl derivatives

AOPP=

advanced oxidation protein products

HAS=

human serum albumin

CRF=

chronic renal failure

HD=

haemodialysis

EPR=

electron paramagnetic resonance

ANS=

ANS-1-anilino-8-naphthalenesulfonic acid

bis-ANS=

4,4′-Bis(1-anilino-8-naphtalenesulfonic acid)

=

dipotassium salt

FITC=

fluorescein isothiocyanate

DPPH=

1,1-diphenyl-2-picrylhydrazyl

DTNB=

5,5′-dithio-bis (2-nitrobenzoic acid)

RBC=

red blood cell

ROS=

reactive oxygen species

TAC=

total antioxidant capacity

SOD=

superoxide dismutase

PBS=

phosphate buffered saline

MDA=

malondialdehyde

NaCl=

sodium chloride

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