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Abstract
The major mechanism of removing cyanide from the body is its enzymatic conversion by a sulfurtransferase, e.g. rhodanese, to the less toxic thiocyanate in the presence of a sulfur donor. Earlier results demonstrated that externally administered encapsulated rhodanese significantly enhances the in vivo efficacy of the given sulfur donor. Present studies are focused on liposomal carrier systems encapsulating rhodanese. Physicochemical properties, e.g. membrane rigidity, size distribution, surface potential, osmolarity, and viscosity, were determined for various liposomal lipid compositions and hydrating buffers to establish in vitro stability and in vivo fate. Lipid composition was also optimized to achieve maximum encapsulation efficiency.
Acknowledgments
This study was supported by NIH: NIAID/USAMRICD Interagency Agreements (Y1-A1-6176-01/02 and A120-B.P2006/7-01), and the ARMY MEDICAL RESEARCH INSTITUTE OF CHEMICAL DEFENSE (Dr Gary Rockwood) under the auspices of the US Army Research Office Scientific Services Program administered by Battelle (Delivery Order 0878, Contract No. DAAD19-02-D-0001, TCN 06-170 and 08284), and the Robert A. Welch Foundation (x-0011) at Sam Houston State University, Huntsville, TX.
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.