Phytic acid-modified manganese dioxide nanoparticles oligomer for magnetic resonance imaging and targeting therapy of osteosarcoma

Abstract

Osteosarcoma is the most common malignant tumor in the skeletal system with high mortality. Phytic acid (PA) is a natural compound extracted from plant seeds, which shows certain antitumor activity and good bone targeting ability. To develop a novel theranostics for magnetic resonance imaging (MRI) and targeting therapy of osteosarcoma, we employed PA to modify manganese dioxide nanoparticles (MnO₂@PA NPs) for osteosarcoma treatment. The MnO₂ NPs oligomer was formed by PA modification with uniformed size distribution and negative zeta potential. Fourier-transform infrared spectroscopy, X-ray diffraction, energy dispersive spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis demonstrated that PA has been successfully modified on MnO₂ NPs, and the structure of MnO₂@PA NPs is amorphous. In vitro experiments demonstrated that MnO₂@PA NPs oligomer can be efficiently internalized by tumor cell, and the internalized NPs can react with H₂O₂ under acid microenvironment to produce Mn²⁺ and O₂. In vivo experiments demonstrated that MnO₂@PA NPs oligomer can passively accumulate in tumor tissue, and the accumulated NPs can produce Mn²⁺ and O₂ for MRI and targeting therapy of osteosarcoma. In conclusion, we prepared a novel bone-targeting nano theranostics for MRI and therapy of osteosarcoma.

Keywords:

• Phytic acid
• manganese dioxide nanoparticles
• osteosarcoma
• magnetic resonance imaging
• targeting therapy

Author contributions

Qian Ju and Rong Huang contributed equally to this study. Qian Ju and Rong Huang performed the main experiments in this manuscript and co-drafted the manuscript. Ruimin Hu, and Junjie Fan participated in the animal experiments and cell experiments. Dinglin Zhang, Rong Li and Jun Ding designed the project and modified the manuscript. All authors have given approval to the final manuscript.

Disclosure statement

The authors declare no conflict of interest.

Ethical approval statement

All animal experiments were performed in accordance with the guidelines for the Care and Use of Laboratory Animals of the Army Medical University (NO. AMUWEC2020121, Chongqing, China). All authors have read the manuscript and consent for publication.

Additional information

Funding

The authors acknowledge support via the Key Support Object of Army Medical University (no. 410301060191) and the Natural Science Foundation of Chongqing (no. cstc2020jcyj-msxmX0469).