Evaluation of RGD Modification on Collagen Matrix

Abstract

Objective: To observe the changes during the process of modificating collagen with Arg-Gly-Asp (RGD) peptide, a series experiments were designed.

Methods: The 3D porous collagen matrices modified with RGD peptide were constructed by lyophilization. The modified and unmodified matrices were characterized by Scanning Electron Microscopy (SEM) and Electron Spectroscopy for Chemical Analysis (ESCA). Fibroblasts were used to evaluate the cell compatibility of the matrices.

Results: In terms of cell growth, the cells attached much better on the modified matrix than on the unmodified one. Compare to the unmodified matrices, the polar groups on the modified matrix increased.

Conclusions: The introducing of specific RGD receptor-mediated adhesion site on matrices obviously enhanced the cells adhesion on collagen matrices.

Keywords:

• Collagen matrix
• RGD
• Surface modification
• Periodate oxidation