Recombinant Hemoglobins as Artificial Oxygen Carriers

Abstract

This paper describes the approaches we have taken to construct a) mutant hemoglobins with different oxygen affinities, and b) mutant hemoglobins and myoglobins that polymerize to high molecular weight aggregates in an effort to prevent extravasation and the associated vasoactivity. In vivo testing indicates that exchange transfusion of polymeric hemoglobins in mice does not result in vasoactivity and that polymeric hemoglobins are effective oxygen carriers to ischemic tissues irrespective of their oxygen affinity and cooperativity.

Keywords:

• Blood substitute
• Heme pocket mutants
• Molecular engineering
• Polymeric hemoglobin
• Surface mutant