Physiological and candidate gene analysis of RG5, a locus associated with a higher ratio of filled grains in rice (Oryza sativa L.)

ABSTRACT

In this report, we investigated physiological and genetic aspect of RG5, a locus increasing the ratio of filled grain in rice (Oryza sativa L). RG5 increased the accumulation of non-structural carbohydrates (NSCs) in the body, and NSCs might contribute to the development of grains after heading. With a chromosome segment substitution line containing ‘Kasalath’ RG5 in the genetic background of the japonica cultivar ‘Koshihikari’, RG5 might enlarge lower leaves and improve NSC supply to the sheath. Through the fine mapping using data plural mutans, we identified NBS-LRR protein (Os05g0380300) as a casual gene for expanding of lower leaves area by RG5. The single nucleotide polymorphism (SNP) of Kasalath caused a premature stop codon and lost function might increase the ratio of filled grains.

GRAPHICAL ABSTRACT

KEYWORDS:

• Leaf sheath
• rice
• source
• yield

Disclosure statement

No potential conflict of interest was reported by the author(s).

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/1343943X.2024.2330729

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This work was supported by MAFF commissioned project study on ‘Smart breeding technologies to Accelerate the development of new varieties toward achieving “Strategy for Sustainable Food Systems, MIDORI’‘ Grant Number JPJ012037.