Abstract
Context
Exocyclic DNA adducts have been shown to be potential biomarkers of cancer risk related to oxidative stress and exposure to aldehydes in smokers. In fact, aldehydes potentially arise from tobacco combustion directly and endogenously through lipid peroxidation.
Objective
This study aims to investigate the relationship between a profile of nine aldehydes-induced DNA adducts and antioxidant activities, in order to evaluate new biomarkers of systemic exposure to aldehydes.
Methods
Using our previously published UPLC-MS/MS method, adducts levels were quantified in the blood DNA of 34 active smokers. The levels of antioxidant vitamins (A, C and E), coenzyme Q10, β-carotene, superoxide dismutase (SOD) and autoantibodies against oxidized low-density lipoprotein were measured.
Results
Adducts induced by tobacco smoking-related aldehydes were quantified at levels reflecting an oxidative production from lipid peroxidation. A significant correlation between SOD and crotonaldehyde-induced adducts (p = 0.0251) was also observed. β-Carotene was negatively correlated with the adducts of formaldehyde (p = 0.0351) and acetaldehyde (p = 0.0413). Vitamin C tended to inversely correlate with acetaldehyde-induced adducts (p = 0.0584).
Conclusion
These results are promising, and the study is now being conducted on a larger cohort with the aim of evaluating the impact of smoking cessation programs on the evolution of adducts profile and antioxidants activities.
Acknowledgments
The authors would like to thank Professor Sophie Pricot for the language proofreading.
Author contributions
Conceptualization, M.L. and M.VDS.; methodology, M.L., H.A. and L.G.; formal analysis, N.H. and M-L.C.; investigation, H.A. and M-L.C.; resources, M.L and L.G.; data curation, M.L. and L.G.; writing—original draft preparation, H.A.; writing—review and editing, M.L.; visualization, H.A.; supervision, M.L. and L.G.; project administration, M.L. and L.G.; funding acquisition, M.L. and H.A. All authors have read and agreed to the published version of the manuscript.
Institutional review board statement
The study was approved by the Scientific and Ethics Committee of CHU UCL Namur, Belgium (NUB: B039201316167).
Informed consent statement
Written informed consent was obtained from all subjects involved in the study.
Disclosure statement
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
Data available on request
The data underlying this article will be shared on reasonable request to the corresponding author.