Multilocus microsatellite typing for Cryptococcus neoformans var. grubii

Ahmed Hanafy Medical Mycology Research Center (former Research Center for Pathogenic Fungi and Microbial Toxicoses), Chiba University, Chiba, Japan

Sirada Kaocharoen Molecular Mycology Research Laboratory, Westmead Millennium Institute, Department of Medicine, University of Sydney Western Clinical School, ICPMR, CIDM, Westmead Hospital, Westmead, New South Wales, Australia; Mycology Unit, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

Alejandro Jover-Botella Molecular Mycology Research Laboratory, Westmead Millennium Institute, Department of Medicine, University of Sydney Western Clinical School, ICPMR, CIDM, Westmead Hospital, Westmead, New South Wales, Australia

Masakazu Katsu Medical Mycology Research Center (former Research Center for Pathogenic Fungi and Microbial Toxicoses), Chiba University, Chiba, Japan

Soji Iida Medical Mycology Research Center (former Research Center for Pathogenic Fungi and Microbial Toxicoses), Chiba University, Chiba, Japan

Takahisa Kogure Medical Mycology Research Center (former Research Center for Pathogenic Fungi and Microbial Toxicoses), Chiba University, Chiba, Japan

Tohru Gonoi Medical Mycology Research Center (former Research Center for Pathogenic Fungi and Microbial Toxicoses), Chiba University, Chiba, Japan

Prof Yuzuru Mikami Medical Mycology Research Center (former Research Center for Pathogenic Fungi and Microbial Toxicoses), Chiba University, Chiba, JapanCorrespondence[email protected]

Wieland Meyer Molecular Mycology Research Laboratory, Westmead Millennium Institute, Department of Medicine, University of Sydney Western Clinical School, ICPMR, CIDM, Westmead Hospital, Westmead, New South Wales, Australia

show all

Abstract

Fifteen randomly selected microsatellites (simple sequence repeats; SSRs), from the H99 Cryptococcus neoformans var. grubii (serotype A) genome, were sequenced, characterized and applied to sequence 87 clinical and environmental C. neoformans var. grubii isolates from 12 different countries based on Multilocus Microsatellite Typing (MLMT). Among the 15 SSR loci, three (designated CNG1, CNG2 and CNG3) were polymorphic, while the remaining 12 SSR loci showed no variations. The specific PCR primers of the polymorphic microsatellites, i.e., CNG1, CNG2 and CNG3, amplified those loci only from strains of C. neoformans (C. neoformans var. grubii, C. neoformans var. neoformans and the AD hybrid) but not from Cryptococcus gattii, suggesting a species-specific association. The three polymorphic microsatellites are useful markers for strain genotyping, population genetic analysis, epidemiological studies, and may be helpful for the diagnosis of cryptococcosis due to C. neoformans.

Keywords

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Multilocus microsatellite typing for Cryptococcus neoformans var. grubii

Information for

Open access

Opportunities

Help and information

Multilocus microsatellite typing for Cryptococcus neoformans var. grubii

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature