Combinatorial hexapeptide ligand libraries (ProteoMiner^™): An innovative fractionation tool for differential quantitative clinical proteomics

Abstract

Blood serum samples are the major source for clinical proteomics approaches, which aim to identify diagnostically relevant or treatment-response related proteins. But, the presence of very high-abundance proteins and the enormous dynamic range of protein distribution hinders whole serum analysis. An innovative tool to overcome these limitations, utilizes combinatorial hexapeptide ligand libraries (ProteoMiner™). Here, we demonstrate that ProteoMiner™ can be used for comparative and quantitative analysis of complex proteomes. We spiked serum samples with increasing amounts (3 μg to 300 μg) of whole E. coli lysate, processed it with ProteoMiner™ and performed quantitative analyses of 2D-gels. We found, that the concentration of the spiked bacteria proteome, reflected by the maintained proportional spot intensities, was not altered by ProteoMiner™ treatment. Therefore, we conclude that the ProteoMiner™ technology can be used for quantitative analysis of low abundant proteins in complex biological samples.

Keywords::

• Proteominer
• sample fractionation
• quantitative serum analysis
• serum profiling

Acknowledgements

Declaration of interest: The authors report no conflict of interest.