A New Method for Screening a-Glucosidase Inhibitors and Application to Marine Microorganisms

Abstract

A new enzyme assay method for screening α-glucosidase inhibitors with rapidity and simplicity was developed. The enzyme-substituted α-glucosidases for this assay was glucoamylase. Samples were spotted or developed on the silica gel plate. The agar solution containing substrate was poured on the plate, and paper impregnated with enzyme was layered on the agar. After incubation, an inhibitory circle would appear around the inhibitor. By using this method, more than 200 strains of marine microorganisms were screened. Among them, three active strains were found to secrete inhibitors in the culture medium.

Keywords: :

• Enzyme assay
• α-glucosidase inhibitor
• marine microorganism