Alterations in Tyrosine Protein Phosphorylation Induced by Matrine in Leukemia Cells

Abstract

Matrine is an alkaloid obtained primarily from roots of Sophora flavescens. Ait. (Leguminosae), an herb used in traditional Chinese medicine to treat a range of disease. So far, the underlying molecular mechanism for the therapeutic effects of matrine has been poorly understood. In this work, we investigated the effects of matrine on tyrosine protein phosphorylation in human cells through immunoblotting. In bcr/abl-positive K562 cells, tyrosine phosphorylations of several proteins are altered in both time-and dose-dependent manners. Such alternations are distinct from those induced by the bcr/abl-targeting inhibitor STI571 and can be blocked by the later. The treatment of bcr/abl-negative lymphocytic and myeloid cell lines by matrine produced different patterns. In Raji cells, the tyrosine phosphorylation was the only protein affected. In U937 cells, no changes were observed. The findings in this work protein the first piece of evidence that matrine can alter the cellular signal transduction network at a molecular level. The observed changes in tyrosine phosphorylation of specific proteins provide important clues for uncovering the molecular mechanism of the therapeutic effects of matrine.

Keywords:

• Leukemia
• matrine
• tyrosine protein phosphorylation