Pharmacological and Immunomodulatory Effects of Aerva lanata. in Daltons Lymphoma Ascites–Bearing Mice

Abstract

Petroleum ether (60–80°C) extract of Aerva lanata. (L.) Juss Ex. was prepared and partially purified by preparative thin-layer chromatography (TLC). The partially purified fraction (PPF) showed significant cytotoxicity against Daltons lymphoma ascites (DLA) tumor cell lines in vitro. and stimulated lymphocyte proliferation in in vitro. and in vivo. conditions. DLA-bearing animals when treated with PPF showed increase in life span compared to control animals. PPF was also found to be hepatoprotective as evidenced from the normal levels of liver marker enzymes compared to the elevated levels of these enzymes in DLA alone inoculated animals. The lipid, hemoglobin, and WBC levels were normal in PPF-treated animals, indicating a low proliferation of tumor cell in peritoneal cavity. Preliminary phytochemical analysis of PPF showed the presence of alkaloids. These results indicate that PPF contains nontoxic immunomodulatory compounds.

Keywords:

• Aerva lanata
• alkaloids
• DLA cells
• lymphocyte
• tumor cells