Anti-inflammatory and Antioxidant Activities of Randia Hebecarpa. and Major Constituents

Abstract

The methanol extract of Randia hebecarpa. Benth. (Rubiaceae) leaves was evaluated for anti-inflammatory activity using carrageenan- and dextran-induced rat paw edema models. Antioxidant activities of the methanol extract and of the fractions resulting from its partition were also measured using the 1,1-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and the linoleic acid peroxidation method. The methanol extract, ethyl acetate fraction, and hydromethanol fraction exhibited percent inhibition of lipid peroxidation comparable to that of commercial antioxidant butylated hydroxytoluene (BHT). Fractionation of the ethyl acetate and hydromethanol fractions through chromatographic methods yielded kaempferol-3, 7-O.-α.-L-dirhamnoside, kaempferol-3-O.-β.-D-galactoside, quercetin-3-O.-β.-D-galactoside, myricetin-3-O.-α.-L-rhamnoside, kaempferol-3-O.-α.-L-rhamnosyl-(1 → 6)-β.-D-galactosyl-7-O.-α.-L-rhamnoside, quinovic acid-3-O.-β.-D-quinovopyranosil-28-O.-β.-D-glucopyranoside, cincholic acid 3-O.-β.-D-quinovopyranosil-28-O.-β.-D-glucopyranoside, and the sugar D-mannitol.

Keywords:

• Anti-inflammatory activity
• flavonoids
• free radical scavenging activity
• lipid peroxidation inhibition
• Randia hebecarpa
• saponins