HPTLC Densitometric Analysis of Orthosiphon stamineus. Leaf Extracts and Inhibitory Effect on Xanthine Oxidase Activity

ABSTRACT

Orthosiphon stamineus. Benth. was cultivated at different locations in Malaysia to compare the xanthine oxidase inhibitory potential of the methanol extracts of the leaf. The extracts showed superoxide scavenging and xanthine oxidase inhibitory activities using in vitro. methods. By means of HPTLC densitometric technique and UV-Vis spectra, sinensetin (SEN) and 3′-hydroxy-5,6,7,4′-tetramethoxyflavone (TMF) concentrations in the extracts were found to vary from 0.09% to 0.36% and 0.02% to 0.10% of the day weight, respectively. The study provided evidence that the leaf of the plant can be useful for studying its anti-gout action.

Keywords:

• HPTLC
• lipid peroxidation
• Orthosiphon stamineus
• sinensetin
• xanthine oxidase