Abstract
From a hexane fraction prepared from the roots of Esenbeckia grandiflora. Mart., effective against fourth instar larvae of Aedes aegypti. L. (LC50 108.23 ppm), eight coumarin derivatives [daphnetin 7-methyl-8-(3,3-dimethylallyl) ether, a mixture containing pimpinellin eight one of its photodimerization product, 5-senecioyl-xanthotoxin, 3-(1′,1′)-dimethylallylcolumbianetin, swietenocoumarin B, isopimpinellin, xanthotoxin, and 5-(1′-hydroxyisopentenyl)bergapten] were isolated in addition to sitosterol and sitostenone. The mixture containing pimpinellin and its photodimerization product was effective against fourth instar larvae (LC50 at 45.77 ppm), and a mixture of pimpinellin and swietenocoumarin B showed a LC50 value of 62.23 ppm. All isolated compounds were identified on the basis of the spectral data including 2D experiments. Furthermore, previously unreported spectral data for some compounds are given.