Determination of Phenolic Acids in Amaryllidaceae Species by High Performance Liquid Chromatography

Abstract

Five Bulgarian Amaryllidaceae species; Pancratium maritimum. L., Sternbergia colchiciflora. W. K., Galanthus nivalis. L., Galanthus elwesii. Hook., and Leucojum aestivum. L., were analyzed for their high-performance liquid chromatography (HPLC) profiles of phenolic acids. An improved HPLC method was developed for the simultaneous determination of seven phenolic acids. Protocatechuic, 4-hydroxybenzoic, vanillic, caffeic, syringic, p.-coumaric, and ferulic acids were identified in the studied species. Protocatechuic, 4-hydroxybenzoic, vanillic, and syringic acids are reported for the first time in Amaryllidaceae. Also, this is the first report on phenolic acid composition in the genus Pancratium., as well as in Galanthus elwesii, Sternbergia colchiciflora., and Pancratium maritimum..

Keywords:

• Amaryllidaceae
• HPLC
• phenolic acids