Anthraquinone Production in Senna alata. Root Cultures

Abstract

Root cultures of Senna alata. (L.) Roxb. were established in liquid B5 medium, supplemented with 0.5 mg l⁻¹ α.-naphthalene acetic acid (NAA) and 1.0 mg l⁻¹ kinetin. The root cultures, which were established from the high-anthraquinone-producing plants, accumulated higher amounts of emodin and chrysophanol than those established from the low-anthraquinone-producing plants and leaves and roots of the intact plants.

Keywords:

• Cassia alata
• chrysophanol
• emodin
• HPLC
• root cultures