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Abstract
This study investigated the influence of serotonergic neurons of the dorsal raphe nucleus (DRN) on the effect of 1,3,7-trihydroxy-2-(3-methylbut-2-enyl)-xanthone, obtained from a hydroethanolic extract (HE) from Kielmeyera coriacea Mart. (Clusiaceae) stems. Intra-DRN microinjection (0.25 μ l/30 s) of xanthone or 5-HT1A ligands and its associations were performed in rats submitted to the forced swimming (FST) and to the open field (OFT) tests. Xanthone (0.3, 0.6 or 0.9 pmol), WAY100635 (5-HT1A antagonist; 0.2, 0.4 or 0.8 nmol) or (+)pindolol (5-HT1A/1B/ßadrenergic antagonist; 0.2, 0.4 or 0.8 nmol) did not alter immobility time in the FST. The 5-HT1A agonist, (+)8-OH-DPAT (0.6, 0.8, or 1.0 nmol) increases the immobility time in higher dose. Associated treatment of WAY100635 (0.8 nmol) or (−) pindolol (0.4 nmol) and xanthone (0.3 pmol), produced an anti-immobility effect, showing a synergic effect. Xanthone (0.3 pmol) abolished the increase on immobility time produced by (+)8-OH-DPAT (1.0 nmol). WAY100635 (0.8 nmol) blocked the increase in immobility time produced by (+)8-OH-DPAT (1.0 nmol). Crossings number in the OFT was not altered by any tested compound or associated treatment. These results suggest that the serotonergic neurons of the DRN, through the 5-HT1A somatodendritic autoreceptors, are involved in the xanthone effects on FST.
Acknowledgements
The authors are thankful to Dra. Rosangela Santana of the Universidade Estadual de Maringá for the statistical support and to Marcos Alberto Trombeli for technical support. This study was supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil).
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.