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Abstract
In our continuous search of biological properties of Senecio species (Compositae), we investigated S. ambiguus subsp. ambiguus (Biv.) DC, S. gibbosus subsp. gibbosus DC, S. leucanthemifolius Poiret, S. inaequidens DC, and S. vulgaris L. for their angiotensin converting enzyme (ACE) inhibitory activity through an in vitro bioassay based on the enzymatic cleavage of the chromophore-fluorophore labelled substrate dansyltriglycine into dansylglycine, which is quantitatively measured by HPLC. Among analyzed extracts, ethyl acetate demonstrated the highest activity with IC50 values of 192.1 and 219.1 μg/mL for S. ambiguus subsp. ambiguus and S. inaequidens, respectively. Flavonoids were detected in these extracts on TLC sprayed with Natural Products reagent - polyethylene glycal reagent (NP/PEG).
Acknowledgements
The authors wish to thank Dr. N.G. Passalacqua and Dr. L. Peruzzi of the Botany Department of University of Calabria, Italy for the identification and collection of the plant materials, and Dr. V. Filippelli for the English revision of this manuscript.
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.