Antinociceptive and anti-inflammatory effects of isolated fractions from Apium graveolens seeds in mice

Abstract

The antinociceptive and anti-inflammatory effects of the aqueous and hexane extracts obtained from Apium graveolens L. (Apiaceae) seeds were evaluated. Formalin and xylene-induced ear edema tests were used in mice. The fractions were administered intraperitoneally at doses of 100-500 mg/kg body weight (BW). Both extracts with the xylene-induced ear edema test showed significant anti-inflammatory activity at all doses as compared with control. Only the hexane fraction reduced the nociception produced by formalin solution in the first phase (0-5 min) at 300, 400, and 500 mg/kg BW, and in the second phase (20-30 min) at 500 mg/kg BW. It is concluded that the hexane fraction has major contribution to the overall antinociceptive activity. Both fractions showed remarkable anti-inflammatory effect which supported the traditional use of Apium graveolens in diseases associated with inflammation.

Keywords::

• Anti-inflammatory activity
• antinociceptive activity
• Apiaceae
• Apium graveolens

Acknowledgements

The researchers extend their sincere gratitude to the deputy of Prand Islamic Azad University for providing experimental tools, and to thank Dr. Gh. Amin for identification of plant material.

Declaration of interest: The authors also wish to thank Ashtian Islamic Azad University for its kind financial support (NO. 1570).