Intensification of marrubiin concentration by optimization of microwave-assisted (low CO₂ yielding) extraction process for Marrubium vulgare using central composite design and antioxidant evaluation

Abstract

Context: Marrubium vulgare Linn (Lamiaceae) was generally extracted by conventional methods with low yield of marrubiin; these processes were not considered environment friendly.

Objective: This study extracts the whole plant of M. vulgare by microwave assisted extraction (MAE) and optimizes the effect of various extraction parameters on the marrubiin yield by using Central Composite Design (CCD).

Materials and methods: The selected medicinal plant was extracted using ethanol: water (1:1) as solvent by MAE. The plant material was also extracted using a Soxhlet and the various extracts were analyzed by HPTLC to quantify the marrubiin concentration.

Results: The optimized conditions for the microwave-assisted extraction of selected medicinal plant was microwave power of 539 W, irradiation time of 373 s and solvent to drug ratio, 32 mL per g of the drug. The marrubiin concentration in MAE almost doubled relative to the traditional method (0.69 ± 0.08 to 1.35 ± 0.04%). The IC₅₀ for DPPH was reduced to 66.28 ± 0.6 μg/mL as compared to conventional extract (84.14 ± 0.7 μg/mL). The scanning electron micrographs of the treated and untreated drug samples further support the results.

Discussion and conclusion: The CCD can be successfully applied to optimize the extraction parameters (MAE) for M. vulgare. Moreover, in terms of environmental impact, the MAE technique could be assumed as a ‘Green approach’ because the MAE approach for extraction of plant released only 92.3 g of CO₂ as compared to 3207.6 g CO₂ using the Soxhlet method of extraction.

Keywords:

• High performance thin layer chromatography
• conventional
• SEM
• 2,2-diphenyl-1-picrylhydrazyl
• green approach

Acknowledgments

The authors acknowledge the University Grant Commission (UGC), an autonomous institute of Ministry of Human Resource Development, Government of India, for providing the financial assistance to create necessary infrastructural and laboratory facilities in Department of Pharmaceutical Sciences, MDU, Rohtak, under special assistance program (SAP) to make this research project feasible.

Disclosure statement

The authors did not declare any conflict of interest.