Mechanism of allergic rhinitis treated by Centipeda minima from different geographic areas

Abstract

Context

The coriander plant Centipeda minima (L.) A. Braun et Aschers (Compositae) is used for the treatment of allergic rhinitis.

Objective

Analyze the difference of the C. minima volatile oil from 7 geographic areas and its therapeutic effect on allergic rhinitis.

Materials and methods

The volatile oils from different geographic areas were extracted and analyzed, the protein and biological pathway for the treatment of allergic rhinitis were predicted by network pharmacology. Established three groups of Sprague-Dawley rat allergic rhinitis models (n = 10). The treatment group was given 100 μL/nostril of 0.1% C. minima volatile oil, the blank and model groups were given the same amount of normal saline. After 15 days, serum inflammatory factors were detected by ELISA. Nasal mucosa tissues were examined by hematoxylineosin staining and immunuhistrochemistry.

Results

There are differences in the content of volatile oil in the seven geographic areas. Experiments showed that the concentration of TNF-α in the serum of the administration group decreased from 63.66 ± 2.06 to 51.01 ± 4.10 (pg/mL), IL-4 decreased from 41.90 ± 3.90 to 28.68 ± 3.39 (pg/mL), IgE decreased from 22.18 ± 1.40 to 17.59 ± 1.60 (pg/mL), IL-2 increased from 314.14 ± 10.32 to 355.90 ± 10.01(pg/mL). Immunohistochemistry showed that compared with the model group, the PTGS2 and MAPK3 proteins in the administration group were significantly reduced.

Discussion and conclusions

C. minima volatile oil is a multi-target and multi-pathway in the treatment of allergic rhinitis, which provides a new research basis and reference for the treatment of allergic rhinitis.

Keywords:

• Network pharmacology
• volatile oil
• molecular docking

Author contributions

Yanzhuo Jia conducted experiments and wrote the manuscript, Xiaofei Zhang and Yajun Shi conceived and designed the research, Junbo Zou and Yao Wang analysed the data, Yulin Liang and Dongyan Guo checked the data, Ming Yang collected the information and revised the manuscript.

Disclosure statement

No conflict of interest has been reported by the author(s).

Additional information

Funding

This project has been supported by the National Natural Science Foundation of China [Grant No. 81703720], Discipline Innovation team Project of Shaanxi University of Chinese Medicine(2019-YL11), the Innovation talents Promotion Program of Shaanxi Province-Science and Technology Innovation team [Grant No. 2018TD005], Project of Science and Technology Department of Shaanxi Province (No.2019SF290), Jiangxi University of Traditional Chinese Medicine Double First-Class Discipline Construction Project (JXSYLXK-ZHYAO008), Major Science and Technology R & D Project in Jiangxi Province (20194ABC28009), The 2017 Open Fund of the Key Laboratory of Modern Chinese Medicine Preparation by the Ministry of Education (2017003).