Effects of Jian Pi Qing Chang Hua Shi decoction on mucosal injuries in a 2,4,6-trinitrobenzene sulphonic acid-induced inflammatory bowel disease rat model

Abstract

Context

Jian Pi Qing Chang Hua Shi decoction (JPQCHSD) has been considered as an effective remedy for the treatment of inflammatory bowel disease (IBD) in Chinese traditional medicine.

Objective

We evaluated the efficacy of JPQCHSD on 2-4-6-trinitrobenzene sulphonic acid (TNBS)-induced IBD rats and the responsible mechanisms.

Materials and methods

Except the rats of the control group (50% ethanol), Sprague–Dawley rats (180 ± 20 g) induced by TNBS (150 mg/kg in 50% ethanol), received water extract of JPQCHSD daily at 0, 9.5, 19, or 38 g/kg for 12 days. The rats were sacrificed, and their colons were removed to evaluate the disease activity index. Malondialdehyde (MDA), superoxide dismutase (SOD), myeloperoxidase (MPO), immunoglobulin A (IgA), tumour necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and nuclear factor-κB were evaluated.

Results

JPQCHSD extract significantly reduced the disease activity index of TNBS-induced colitis with a median effective dose (ED₅₀) of 26.93 g/kg. MPO and MDA were significantly reduced in the 19 and 38 g/kg groups (ED₅₀ values 37.38 and 53.2 g/kg, respectively). The ED₅₀ values for the increased SOD and IgA were 48.98 and 56.3 g/kg. ED₅₀ values for inhibition of TNF-α, IL-1β, and IL-6 were 32.66, 75.72, and 162.06 g/kg, respectively.

Discussion

JPQCHSD promoted mucosal healing in IBD rats via its anti-inflammation, immune regulation, and antioxidation properties.

Conclusions

JPQCHSD has healing function on IBD. Further clinical trials are needed to demonstrate its efficacy and tolerance to IBD.

Keywords:

• Superoxide dismutase
• malondialdehyde
• myeloperoxidase
• interleukin-1β
• tumour necrosis factor-α
• immunoglobulin A
• nuclear factor-κB

Disclosure statement

The authors declare that they have no competing interests regarding this manuscript.

Data availability statement

All data generated in this study to support the conclusions are included in this article.

Additional information

Funding

This work was supported by the National Nature Science Foundation of China under Grant nos. 81774257 and 81873244; Beijing Municipal Natural Science Foundation under Grant no. 7172098; Second Batch of Special Research Projects on Business Construction of the National Clinical Research Base of Traditional Chinese Medicine under Grant no. JDZX2015085 and Fund for Beijing Science & Technology Development of TCM no. JJ2018-30.