Expansion of megakaryocyte progenitors from cryopreserved leukocyte concentrates of human placental and umbilical cord blood in short-term liquid culture

N Sasayama Cell Processing Department, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.

I Kashiwakura Laboratory of Radiopharmaceutical Sciences, Hokkaido College of Pharmacy, Otaru, Japan.

Y Tokushima Cell Processing Department, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.

S Wada Cell Processing Department, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.

M Murakami Cell Processing Department, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.

Y Hayase Laboratory of Radiopharmaceutical Sciences, Hokkaido College of Pharmacy, Otaru, Japan.

Y Takagi Laboratory of Radiopharmaceutical Sciences, Hokkaido College of Pharmacy, Otaru, Japan.

T A Takahashi Cell Processing Department, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.

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Abstract

Long-term severe thrombocytopenia following human placental and umbilical cord blood (CB) transplantation is a significant clinical problem. We studied the ex vivo expansion of megakaryocytic progenitor cells (CFU-Meg) from cryopreserved/thawed leukocyte concentrates (LC) of CB prepared by the Tokyo Cord Blood Bank protocol. The LC cells were cultured in serum-free culture medium supplemented with a combination of early-acting cytokines including thrombopoietin (TPO), flt3-ligand (FL), and stem cell factor (SCF). Combination of TPO plus FL, TPO plus SCF, and all of these cytokines together resulted in 8.9-, 7.7-, and 8.4-fold increases in CFU-Meg, respectively, by Day 5 of culture. Our results showed that this simple expansion strategy has the potential for expanding CFU-Meg from cryopreserved/thawed LC cells from CB.

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Expansion of megakaryocyte progenitors from cryopreserved leukocyte concentrates of human placental and umbilical cord blood in short-term liquid culture

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Expansion of megakaryocyte progenitors from cryopreserved leukocyte concentrates of human placental and umbilical cord blood in short-term liquid culture

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