In vitro effects of some drugs on human red blood cell glucose-6-phosphate dehydrogenase enzyme activity

Abstract

In vitro effects of omeprazole, morphine sulphate, remifentanyl, ketamine and vankomycin were investigated on human red blood cell glucose-6 phosphate dehydrogenase (G-6PD) (E.C. 1.1.1.49) enzyme activity purified from human red blood cell by 2′, 5′ADP-Sepharose 4B affinity gel. The obtained I₅₀ values of omeprazole, morphine, remifentanil, ketamine and vankomycin were 3.24, 43.58, 97.6, 64.16 and 0.903 mM, respectively and the K_i constants for omeprazole, morphine and vankomycin were 8.22 ± 2.055, 25.93 ± 6.482, and 2.71 ± 0.677 mM, respectively and they were non competitive inhibitors.

Keywords::

• Glucose-6-phosphate dehydrogenase
• human red blood cell
• inhibition