Synthesis, lipophilicity study and in vitro evaluation of some rodenticides as acetylcholinesterase reversible inhibitors

Abstract

The anti-AChE activity of phosphoramidates has been noticed for many years. Because of the wide application of phosphoramidates in recent years, there has been a continuing research for synthesis, purification and identification of effective and safe derivatives. In this study some rodenticides with the general formula Me₂NP(O)(p-OC₆H₄-X)₂, where X = H, CH₃, Cl, have been synthesized in water (without organic solvent) and characterized by ³¹P, ³¹P {¹H}, ¹³C and ¹H NMR spectroscopy. Since lipophilicity has been recognized for its importance in QSAR studies, efforts have been made to determine the logP values. The ability of these rodenticides to inhibit human acetylcholinesterase (hAChE) has been predicted with PASS (Prediction of Activity Spectra for Substances) software (version 1.917) and then has been evaluated by a modified Ellman's assay and spectrophotometric measurements.

Keywords::

• Phosphoramidate
• rodenticide
• lipophilicity
• acetylcholinesterase
• inhibition

Acknowledgements

We are sincerely grateful to the Department of Biology for providing us with a biochemistry lab and thankful to the New Ideas Research Institute for the gift of AChE.