Tyrosinase inhibitory components from Aloe vera and their antiviral activity

Abstract

A new compound, 9-dihydroxyl-2'-O-(Z)-cinnamoyl-7-methoxy-aloesin (1), and eight known compounds (2–9) were isolated from Aloe vera. Their structures were elucidated using 1D/2D nuclear magnetic resonance and mass spectra. Compound 9 exhibited reversible competitive inhibitory activity against the enzyme tyrosinase, with an IC₅₀ value of 9.8 ± 0.9 µM. A molecular simulation revealed that compound 9 interacts via hydrogen bonding with residues His244, Thr261, and Val283 of tyrosinase. Additionally, compounds 3 and 7 were shown by half-leaf assays to exhibit inhibitory activity towards Pepper mild mottle virus.

Keywords:

• Aloe vera
• molecular docking
• Pepper mild mottle virus
• tyrosinase
• Xanthorrhoeaceae

Disclosure statement

This study was supported partly by a basic research project (PJ010878) of National Institute of Horticultural and Herbal Science, RDA.