A new colorimetric DPPH^• scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs

Abstract

2,2-Diphenyl-1-picrylhydrazyl (DPPH^•) radical scavenging, the most commonly used antioxidant method with more than seventeen thousand articles cited, is very practical; however, as with most assays, it has the major disadvantage of dependence on a spectrophotometer. To overcome this drawback, the colorimetric determination of the antioxidant activity using a scanner and freely available Image J software was developed. In this new method, the mixtures of solutions of DPPH^• and standard antioxidants or extracts of common medicinal herbs were dropped onto TLC plates, after an incubation period. The spot images were evaluated with Image J software to determine CSC₅₀ values, the sample concentrations providing 50% colour reduction, which were very similar with the SC₅₀ values obtained with spectrophotometric method. The advantages of the new method are the use of lower amounts of reagents and solvents, no need for costly spectrophotometers, and thus significantly lowered costs, and convenient implementation in any environment and situation.

Keywords:

• TLC plates
• Image J software
• 2,2-diphenyl-1-picrylhydrazyl
• antioxidant

Acknowledgements

We gratefully acknowledge Karadeniz Technical University research fund (Projects No 666 and 907) for the financial support.

Disclosure statement

The authors report no declarations of interest.

Additional information

Funding

We gratefully acknowledge Karadeniz Technical University research fund (Projects No 666 and 907) for the financial support.