New dammarane-type triterpenoids from hydrolyzate of total Gynostemma pentaphyllum saponins with protein tyrosine phosphatase 1B inhibitory activity

Abstract

Protein tyrosine phosphatase 1B (PTP1B) is a key factor and regulator of glucose, lipid metabolism throughout the body, and a promising target for treatment of type 2 diabetes mellitus (T2DM). Gynostemma pentaphyllum is a famous oriental traditional medicinal herbal plant and functional food, which has shown many beneficial effects on glucose and lipid metabolism. The aim of the present study is to assess the inhibitory activity of five new and four known dammarane triterpenoids isolated from the hydrolysate product of total G. pentaphyllum saponins. The bioassay data showed that all the compounds exhibited significant inhibitory activity against PTP1B. The structure-activity relationship showed that the strength of PTP1B inhibitory activity was mainly related to the electron-donating group on its side chain. Molecular docking analysis suggested that its mechanism may be due to the formation of competitive hydrogen bonding between the electron-donating moiety and the Asp48 amino acid residues on the PTP1B protein.

Keywords:

• Gynostemma pentaphyllum
• dammarane triterpenoids
• protein tyrosine phosphatase 1B
• phytochemistry

Disclosure statement

The authors report no conflicts of interest.

Additional information

Funding

This work was financially supported by the National Natural Science Foundation of China (NSFC, Nos. 82260843, 82060649, 82160740), Guizhou Engineering Research Centre of Industrial Key-technology for Dendrobium Nobile (QJJ [2022]048, QJJ [2022]006), Zunyi of China (QKHPTRC [2020]-16, ZSKHHZZ (2021)290).