The levels of peripheral blood TNF-α, Decorin and neutrophils MAPK1 mRNA levels of patients with preeclampsia and their clinical significance

Abstract

Objective

This study analyzed the levels of peripheral blood tumor necrosis factor-α (TNF-α), Decorin (DCN) and Mitogen-activated protein kinase 1 (MAPK1) mRNA in neutrophils of patients with preeclampsia and their correlations, in order to provide more thoughts on the diagnosis and treatment of clinical patients.

Methods

81 patients with preeclampsia who had regular prenatal checkups and delivered in our hospital from June 2020 to April 2022 were analyzed, including 26 patients with early-onset and 55 patients with late-onset, and 50 pregnant women with normal pregnancy who had prenatal checkups and delivered in our hospital during the same period were selected as the control group. Record the clinical data of patients, record the expression of peripheral blood TNF-α, DCN and neutrophils MAPK1 mRNA of patients with early-onset, late-onset and the control group, and record the correlation between DCN level, MAPK1 mRNA expression and TNF-α level of patients with preeclampsia.

Results

The diastolic and systolic blood pressures were significantly higher in early-onset and late-onset patients, and the gestational week of delivery was significantly lower in early-onset and late-onset patients, respectively (p < .05); there was no statistically significant difference in the average age, BMI, average pregnancy time, and average births between the three groups (p > .05). The expressions of peripheral blood TNF-α, DCN, and neutrophils MAPK1 mRNA of the early-onset and late-onset groups were all higher than those in the control group (p < .05); and the expressions of TNF-α, DCN, and MAPK1 mRNA in the peripheral blood of the early-onset group were all higher than those in the late-onset group (p < .05); Pearson correlation analysis showed that DCN level and TNF-α level in patients with preeclampsia were positively correlated (r = 0.98639, p < .05); Neutrophils MAPK1 mRNA expression and TNF-α level were positively correlated (r = 0.9611, p < .05).

Conclusion

TNF-α, DCN and neutrophils MAPK1 mRNA were all highly expressed in the peripheral blood of patients with pre-eclampsia and were more significantly elevated in patients with early-onset preeclampsia, and the expression levels of DCN and MAPK1 mRNA were positively correlated with TNF-α levels. It is possible that all three factors are involved in the pathogenesis of preeclampsia, and are expected to be used as indicators for early prediction and diagnosis.

Keywords:

• Preeclampsia
• tumor necrosis factor-α
• Decorin
• mitogen-activated protein kinase

Ethical approval

This study was approved by the Ethics Committee of Jinshan Branch of Shanghai Sixth People’s Hospital. Written informed consent was obtained from the parents for publication of clinical details, clinical images, and videos.

Authors’ contributions

YNZ finished the conception, design, and implementation of the project, and prepared the manuscript. GPG assisted in the conception and design of the project, provided technical assistance. All authors approved the final manuscript and agreed to be accountable for all aspects of the work.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statment

The datasets supporting the conclusions of this article are included within the manuscript. The demanders may contact the corresponding author.

Institutional review board statement

Ethical review and approval were waived for this study (approval number: jszxyy202233), due to the study not meeting criteria for research involving human subjects.

Additional information

Funding

This research supported by Jinshan District Medical and Health Science and Technology Innovation Fund Project [grant No: 2022-WS-06] and Jinshan Branch of Shanghai Sixth People's Hospital Research Fund [grant No: 2021-1].