Abstract
The present study investigated the effect of fenugreek seed polyphenolic extract (FPEt) on ethanol-induced protein expression in Chang liver cells. Cells were incubated with either 30 mM EtOH alone or together in the presence of FPEt for 24 h. Cells were harvested and assessed for expression of alcohol metabolizing enzymes-alcohol dehydrogenase (ADH2 isoform), aldehyde dehydrogenase (ALDH2 isoform), cytochrome P450 (CYP2E1), the electron transport component (cytochrome-c), and the heat shock proteins. The expression of ADH2, ALDH2, and CYP2E1 were upregulated, whereas the expression of cytochrome-c was downregulated in the ethanol-treated cells. The expression of cellular heat shock proteins-HSP70, HSC70, HSC92, and mitochondrial protein mtHSP70 were induced in ethanol-treated Chang liver cells. FPEt modulated the protein expression changes induced by ethanol and had no effect when incubated with normal Chang liver cells. FPEt might exert cytoprotective action on ethanol-induced liver cell damage, possibly by enhancing cellular redox status.
Financial support to Mr S. Kaviarasan, one of the authors, in the form of Senior Research Fellowship (SRF) by the Indian Council of Medical Research (ICMR), New Delhi, is gratefully acknowledged. The authors wish to thank Professor Dr William F. Bosron, Dr Henry Weiner, Dr Lesley McLaughlin, Dr Ronald Jemmerson, Dr Srini V. Kaveri, Dr Mike Ryan, Dr Brooke Bevis, and Dr Toft David O, for providing antibodies.