Evidence that the V832M E-Cadherin Germ-Line Missense Mutation does not Influence the Affinity of α -Catenin for the Cadherin/Catenin Complex

Matthew W. Curtis Departments of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska

Quan P. Ly Departments of Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska; Departments of Surgery, University of Nebraska Medical Center, Omaha, Nebraska

Margaret J. Wheelock Departments of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska; Departments of Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska; Departments of Oral Biology, University of Nebraska Medical Center, Omaha, Nebraska; Departments of Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska

Keith R. Johnson Departments of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska; Departments of Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska; Departments of Oral Biology, University of Nebraska Medical Center, Omaha, Nebraska; Departments of Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska

Abstract

Mutations in E-cadherin are associated with a number of diseases, and have been shown to contribute to disease progression. In particular, 50% of hereditary diffuse gastric cancer cases have inactivating mutations in the E-cadherin gene. An interesting mutation near the beta-catenin-binding site on the cytoplasmic domain of E-cadherin (V832M) was recently reported that produces full-length protein, but exhibits decreased binding of α -catenin to the cadherin/catenin complex. The study was done by transfecting mutant E-cadherin into Chinese hamster ovary fibroblast cells. Here we show that the previously reported characteristics of this mutation do not apply to human epithelial cells expressing this mutant protein and suggest that the mechanism whereby the V832M mutation in human E-cadherin promotes gastric cancer is not yet understood.

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Evidence that the V832M E-Cadherin Germ-Line Missense Mutation does not Influence the Affinity of α -Catenin for the Cadherin/Catenin Complex

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Evidence that the V832M E-Cadherin Germ-Line Missense Mutation does not Influence the Affinity of α -Catenin for the Cadherin/Catenin Complex

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