https://orcid.org/0000-0003-2790-2365
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Abstract
Introduction
We examined the relationship between proinflammatory cytokines that occur in the inflammatory reaction in the intestine in Hirschsprung disease (HD) and Hirschsprung-associated enterocolitis (HAEC).
Methods
Thirty cases (M:27, F:3) operated on due to HD. The cases were divided into three groups: group 1 with pre and post operative EC, group 2 with post-operative, and group 3 with pre-operative EC. The intestinal segments were evaluated by immunohistochemistry for interleukin 1 beta (IL-1ß), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6).
Results
IL-1β staining was significantly higher in the ganglionic zone of groups with enterocolitis compared to the control group (p = 0.012). TNF-α staining in the transitional zone of Group 3 and IL-1β staining in the ganglionic zone of Group 1 was significantly higher than the control group (p = 0.030, p = 0.020).
Conclusion
In our study, older age at diagnosis and more than 20% IL-1ß staining in the ganglionic segment were found to be risk factors for HAEC. It is noteworthy that the increase in IL-1ß can be associated with HAEC.
Acknowledgments
We thank Istanbul University-Cerrahpaşa Scientific Research Projects Unit for supporting our study (Project ID: 36569).
Thanks for the preprint (doi:10.21203/rs.3.rs-2666915/v1) available at Reseach Square.
Author contributions
Conceptualization: Yalım Benibol, Ayse Mine Onenerk, Rahsan Ozcan. Methodology: Nil Comunoglu, Ali Ekber Hakalmaz, Gonca Topuzlu Tekant, Yalım Benibol. Formal analysis and investigation: Yalım Benibol, Ayse Mine Onenerk, Rahsan Ozcan, Nil Comunoglu. Writing—original draft preparation: Yalım Benibol, Ayse Mine Onenerk, Rahsan Ozcan, Ali Ekber Hakalmaz. Writing—review and editing: Yalım Benibol, Nil Comunoglu, Gonca Topuzlu Tekant.
Disclosure statement
No potential conflict of interest was reported by the authors.
Funding
The study was supported by Istanbul University-Cerrahpaşa Scientific Research Projects Unit.