Histopathological and biochemical alterations in non-diabetic and diabetic rats following acrylamide treatment

Abstract

Acrylamide (50 mg/kg) was daily administered intraperitoneally to normal and diabetic rats for 2 weeks. Its impact was evaluated by histopathology, western blot, and serum biochemical analysis. Levels of serum alanine aminotransferase, blood urea nitrogen, uric acid, creatinine, and lactate dehydrogenase in acrylamide-treated diabetic rats were higher than in acrylamide-treated non-diabetic rats. Also, liver and kidney tissue lesions induced by acrylamide were more severe in diabetic rats than in normal rats. The higher toxicity of acrylamide to diabetic rats may be caused by the overexpression of CYP2E1 monooxygenase, potentially resulting in the metabolic formation of the more reactive glycidamide.

Keywords:

• Acrylamide
• diabetes
• CYP2E1
• liver
• kidney

Acknowledgment

The authors are thankful to Mashhad University of Medical Sciences for financial support. The results in this paper were part of thesis of Asieh Karimani.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors are thankful to the Vice Chancellor of Mashhad University of Medical Sciences for financial support.