Antioxidant, antiepileptic, and anticholinergic properties of 4′,5,7-Trihydroxy-3,6-dimethoxyflavone as natural phenolic compound: a toxicology approach

Abstract

In this work, the antioxidant properties of 4′,5,7-trihydroxy-3,6-dimethoxyflavone using several bioanalytical assays is investigated in vitro. These methods are Cu²⁺-Cu⁺ reducing, Fe³⁺-Fe²⁺reducing, DPPH^• scavenging, and ABTS^•+ scavenging. The IC₅₀ values of the 4′,5,7-trihydroxy-3,6-dimethoxyflavone for DPPH^• and ABTS^•+ scavenging assays were 9.36 and 3.55 μg m^–1, respectively. Additionally, 4′,5,7-trihydroxy-3,6-dimethoxyflavone compound recorded best inhibitory effects against human carbonic anhydrase (hCA) I and II isoenzymes, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) enzymes. 4′,5,7-Trihydroxy-3,6-dimethoxyflavone had K_i value of 389.56 ± 29 nM against hCA I and 306.90 ± 41 nM toward hCA II, respectively. Also, the K_i values of AChE and BChE were found as 1005.37 ± 117 nM and 814.22 ± 87 nM, respectively.

Keywords:

• 4′,5,7-Trihydroxy-3,6-dimethoxyflavone
• antioxidant activity
• metabolic enzymes
• enzyme inhibition
• acetylcholinesterase

Disclosure statement

No potential conflict of interest was reported by the authors.