Human umbilical cord-derived mesenchymal stem cells conditioned medium ameliorates CCl₄-induced liver fibrosis through regulation of expression and activity of liver lysyl oxidase

Abstract

The effects of human umbilical cord blood mesenchymal stem cells conditioned medium (hMSC-CM) on Lysyl oxidase (LOX) family gene expression and proteins were evaluated. Fifty-four male Wistar rats were divided into nine groups. The control groups received olive oil and phosphate-buffered saline (PBS) while untreated groups received CCl₄. The treated groups received hMSC-CM after the 4th week of CCl₄ injection. LOX family expression and proteins were evaluated by RT-PCR and western blot. CCl₄ enhanced significantly the expression of LOX genes and proteins. hMSC-CM significantly decreased the expression of the LOX genes and proteins. hMSC-CM decreased cross-linked collagen in the treated groups. hMSC-CM improved liver fibrosis through decreased LOX family gene expression.

Keywords:

• Liver fibrosis
• Lysyl oxidase
• conditioned medium
• genes expression
• proteins

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was funded by Vice-chancellor for Research and Technology, Hamadan University of Medical Sciences [9605033007].