Medicago sativa ameliorated cyclophosphamide-induced thrombocytopenia and oxidative stress in rats

Abstract

Effect of Medicago sativa (MS) hydro-ethanolic extract against the cyclophosphamide (CP)-induced toxicity was evaluated in rats grouped as: Control; CP (100 mg/kg subcutaneous, day 1–3); and CP+ MS100, 200, and 400 mg/kg/day, orally for 14 days. Hematologic, lipid, hepatic and oxidative stress markers were analyzed. Platelet, WBC, RBC, Hb and Hct in the CP group were lower than the control. The MS200 and MS400 groups had lower lipids, liver enzymes, nitrite and malondialdehyde levels but higher platelet, WBC, thiol, superoxide dismutase and catalase levels than the CP group. MS ameliorated CP-induced thrombocytopenia, oxidative stress, and increases in serum lipids and hepatic enzymes.

Keywords:

• Cyclophosphamide
• Medicago sativa
• Thrombocytopenia
• Oxidative stress
• Liver

Acknowledgements

The results mentioned in this article are part of an M.Sc. thesis.

Author contributions

Zahra gholamnezhad: designed and supervised the study, analyzed the data and prepared the manuscript. Vajihe Rouki: conducted the experiments and analyzed the data. Ramin Rezaee: advised the study and revised manuscript. Mohammad Hossein Boskabady: advised the study and revised manuscript. All authors approved the final version of the manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The authors received the financial support from the Vice Presidency of Research of Mashhad University of Medical Sciences (grant number 941500).