Quality assessment of the preparation and storage of leukocyte-depleted pooled platelet concentrates

ABSTRACT

Objective:

To explore the feasibility of using a disposable platelet storage bag containing a leukocyte filter to prepare leukocyte-depleted pooled platelet concentrates with the buffy coat method.

Methods:

150 bags of whole blood samples (400 mL/bag) were stored overnight at 22 ± 2°C, and buffy coats were separated on Day 2, then 5 units of ABO homotypic buffy coat and 1 unit of plasma were pooled into a disposable platelet storage bag containing a leukocyte filter to prepare leukocyte-depleted pooled platelet concentrates and stored in a Platelet Agitator. On Day 2, 4, 5 and 7 after the collection of whole blood, platelet content, pH value, pO₂, pCO₂, glucose (GLU), ATP, and other quality indicators were measured.

Results:

The quality indicators of leukocyte-depleted pooled platelet concentrates met the requirements for leukocyte-depleted aphaeresis platelets in the Chinese national standard Quality Requirements for Whole Blood and Blood Components (GB18469-2012). With the prolongation of storage time, MPV and PDW of platelets gradually increased, pH value, bicarbonate, and GLU gradually decreased, LA, LDH, and ATP gradually increased, pO₂ slightly increased, pCO₂ decreased, and HSR had no significant change. ESC decreased significantly on Day 7, CD62p decreased first and then increased, sP-selectin and GP V increased first and then decreased, but the results on Day 7 were higher than those on Day 2.

Conclusion:

The quality of leukocyte-depleted pooled platelet concentrates prepared by the buffy coat method using disposable platelet storage bags containing a leukocyte filter was comparable to that of leukocyte-depleted apheresis platelets, and could be used clinically.

KEYWORDS:

• Buffy coat method
• leukocyte-depleted pooled platelet concentrates
• platelet quality
• leukocyte filtration
• apheresis platelets
• quality indicators
• in vitro storage quality
• platelet storage bag

Acknowledgements

The authors are grateful to all of the participants and contributors.

Author contributions

Feng Chen and Xiaoqing Dai: design & statistical analysis; Feng Chen and Azhong Li: wrote the first draft of the manuscript. Wei Hu: provision of study material or patients; Feng Chen and Yinhong Zheng: writing-review & editing. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.