Identification of HIST1H2BH as the hub gene associated with multiple myeloma using integrated bioinformatics analysis

ABSTRACT

Objectives

Identifying the specific biomarkers and molecular signatures of MM might provide novel evidence for MM prognosis and targeted therapy.

Methods

Bioinformatic analyses were performed through GEO and TCGA datasets. The differential expression of HIST1H2BH in MM sample was validated by the qRT-PCR. And the CCK-8 assay was performed to detect the proliferation activity of HIST1H2BH on MM cell lines.

Results

A total of 793 DEGs were identified between bone marrow plasma cells from newly diagnosed myeloma and normal donors in GSE6477. Among them, four vital genes (HIST1H2AC, HIST1H2BH, CCND1 and TCF7L2) modeling were constructed. The increased HIST1H2BH expression was correlated with worse survival of MM based on TCGA datasets. The transcriptional expression of HIST1H2BH was significantly up-regulated in primary MM patients. And knockdown HIST1H2BH decreased the proliferation of MM cell lines.

Conclusions

We have identified up-regulated HIST1H2BH in MM patients associated with poor prognosis using integrated bioinformatical methods.

KEYWORDS:

• Multiple myeloma
• bioinformatics analysis
• independent prognostic factor‌
• differentially expressed gene
• hub genes
• epigenetic regulation
• H2B protein family
• histone modification

Disclosure statement

No potential conflict of interest was reported by the author(s).

Authors’ contributions

Conceived and designed the experiments: W.X.Z., X.C. and Y.Y.Z. Performed the experiments: W.X.Z., X.C., Y.Y.Z., Q.W., J.F., Z.W., D.X.W., Z.C.W. Analyzed the data: J.X.T., S.Y.Q. Contributed reagents/materials/analysis tools: W.X.Z., X.C. and Y.Y.Z. Wrote the paper: W.X.Z., X.C. and Y.Y.Z. The authors read and approved the final manuscript.

Limitation of the present study

The major limitation of the present study is the small sample size. The results would be more reliable with more enrolled MM patients.

Ethics approval and consent to participate

The present study was approved by the Ethics Committee of the Affiliated Hospital of Qingdao University. The ethics serial number is QYFY WZLL 28115.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Additional information

Funding

The present study was funded by the Natural Science Foundation of Shandong Province, ZR2020MH314 (to Xian Chen), the Natural Science Foundation of Shandong Province, ZR2021MH311 (to Yunyuan Zhang).