Genetic diversity and population structure of Canarian chicken using microsatellite DNA markers

Abstract

The Canary Islands have historically been a crossway among at least three continents; therefore, the genetic influences on their local animal breeds have been extremely diverse. In the Canarian chicken population, genetic diversity is evident but it has never been studied. The aim of this study was to assess the population structure and genetic diversity within the Canarian chicken population, as well as between the Canarian chicken population, the Spanish local populations, and the commercial chicken populations with microsatellites, with a view to reinforce the official recognition of the breed, for the design and development of a conservation program. Blood samples were collected at random from 198 animals of the Canarian and compared with Spanish local and commercial strains, in order to determine differentiation and genetic relatedness. The five phenotypic varieties of Canarian chicken, the population structure and genetic diversity within the Canarian chicken population had a higher unbiased expected heterozygosity than the observed heterozygosity. Comparing the Canarian vs other local Spanish breeds and commercial strains, FST was relatively high (0.179) (0.164 – 0.195), the neighborhood network showed that the Canarian varieties did not cluster with the other Spanish breeds. The STRUCTURE, confirmed that the Rubilana variety differed from the other four Canary Islands. We conclude that the Canary Islands chicken population shows a differentiated genetic profile, versus other Spanish and cosmopolitan breeds. The theory that the existence of genetic varieties is based on the color of feathers was definitively discarded, except in the Rubilana population, which could be admitted as a genetically different variety.

HIGHLIGHTS

• The Rubilana chicken variety appeared to be genetically differentiated from the other Canarian varieties.

• The Canarian varieties did not cluster with the other Spanish breeds.

• The theory of the existence of genetic varieties within the breed is discarded, except for the Rubilana population.

Keywords:

• microsatellites
• conservation
• Rubilana
• genetic differentiation

Acknowledgements

This study was included in Fundación Caja Canarias and Obra Social La Caixa under Grant Project 2018PATRI31 and funded by Canarian Government and Canary Islands Institute for Agricultural Research (ICIA). This work would not have been possible if it had not been for the support and assistance of the PAIDI AGR 218 research group and Animal Breeding Consulting.

Data availability statement

Data will be made available from the corresponding author A.M.C.V. upon reasonable request.

Disclosure statement

No potential conflict of interest was reported by the authors.