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ABSTRACT
The influence of cumulus cells (CC) on the lipid profile of bovine oocytes matured in two different lipid sources was investigated. Cumulus-oocyte complexes (COC) or denuded oocytes (DO) were matured in tissue culture medium (TCM) supplemented with fetal bovine serum (FBS) or serum substitute supplement (SSS). Lipid profiles of TCM, serum supplements, immature CC and oocyte (IO), and in vitro-matured oocytes from COC and DO were then analyzed by matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and submitted to partial least squares-discriminant analysis (PLS-DA). The developmental competence of such oocytes was also assessed. Differences in lipid composition were observed between two types of sera and distinctly influenced the lipid profile of CC. As revealed by PLS-DA, the abundance of specific ions corresponding to triacylglycerols (TAG) or phospholipids (PL) were higher in COC compared to DO both supplemented with FBS or SSS and to some extent affected the subsequent DO in vitro embryo development. DO exposed to SSS had however a marked diminished ability to develop to the blastocyst stage. These results indicate a modulation by CC of the oocyte TAG and PL profiles associated with a specific cell response to the serum supplement used for in vitro maturation.
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Declaration of interest
The authors report no conflicts of interest.
Additional information
Notes on contributors
Alessandra A. Vireque
Designed the study, data collection, performed the experiments, analyzed and interpreted the data, and wrote the manuscript: AAV; Responsible for the MALDI-MS analyses, interpretation of MALDI-MS data, helped in the critical discussion of results, and revised the manuscript: AT, KRAB, FNS, MNE; Performed data collection, in vitro embryo production experiments, analyzed and interpreted the data, and wrote the manuscript: JGVG, DRA, ACB; Interpreted the data and critically revised the manuscript: MFSS, RAF; Conceived and designed the study, analyzed the data, and wrote the manuscript: ACJSRS.